Complete genomic characterization of two European-genotype porcine reproductive and respiratory syndrome virus isolates in Fujian province of China

作者

Liu, Jian-Kui; Wei, Chun-Hua; Dai, AI-Ling; 更多内容

期刊:ARCHIVES OF VIROLOGY ISSN:0304-8608 , 年:2017 . 卷:162 . 期:3 页码:823-833

语种: English

原文链接:http://dx.doi.org/10.1007/s00705-016-3136-9

摘要

Porcine reproductive and respiratory syndrome (PRRS) is considered one of the most devastating swine diseases worldwide, resulting in immense economic losses. PRRS virus (PRRSV) is divided into two major genotypes, European (type 1) and the North American (type 2). Type 1 PRRSV have recently emerged in Fujian province (South China), and this might have a significant impact on the Chinese pig industry. From 2013 to 2014, two type 1 PRRSV strains, named FJEU13 and FJQEU14, were isolated from piglets and sows with respiratory problems and reproductive disorders in Fujian province. The full genome length of the two isolates was 14,869-15,062 nucleotides (nt), excluding the poly(A) tail. These isolates shared 86.0-89.9% sequence identity with the prototypic strains Lelystad virus (LV) and 82.8-92% with Chinese type 1 PRRSV strains, but only 59.9-60.1% with the North American reference strain VR-2332. However, they were 82.9% identical to each other. Nonstructural protein 2 (Nsp2) and ORF3-ORF5 were the most variable regions when compared to other type 1 PRRSV strains. Nsp2 and ORF3 contained multiple discontinuous deletions and a 204-bp deletion in NSP2 in isolate FJQEU14, which has never been described in other Chinese type 1 PRRSV strains. All of these results might be useful for understanding the epidemic status of type 1 PRRSV in China.

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附加关键词:
GENETIC DIVERSITY; UNITED-STATES; NORTH-AMERICAN; NSP2; PROTEIN; PRRSV; SEQUENCE; STRAINS; REGION; IDENTIFICATION

作者信息

通讯作者:
     Yang, XY , Longyan Univ, Coll Life Sci, Longyan 364012, Fujian, Peoples R China.; Yang, XY , Fujian Prov Key Lab Prevent & Control Anim Infect, Longyan 364012, Fujian, Peoples R China.
作者机构:
     [1] [ Liu, Jian-Kui; Wei, Chun-Hua; Dai, AI-Ling; Fan, Ke-Wei; ︾ ] Longyan Univ, Coll Life Sci, Longyan 364012, Fujian, Peoples R China.
     [2] [ Liu, Jian-Kui; Wei, Chun-Hua; Dai, AI-Ling; Fan, Ke-Wei; ︾ ] Fujian Prov Key Lab Prevent & Control Anim Infect, Longyan 364012, Fujian, Peoples R China.
     [3] [ Liu, Jian-Kui; Luo, Man-Lin ] South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.
作者邮箱:
     liujiankui99@126.com
     lyyxy1988@126.com