Age patterns of Cryptosporidium especies and Giardia duodenalis in dairy calves in Egypt

作者

Naguib, Doaa; El-Gohary, Adel H; Mohamed, Amro A; 更多内容

期刊:PARASITOLOGY INTERNATIONAL ISSN:1383-5769 , 年:2018 . 卷:67 . 期:6 页码:736-741

语种: English

原文链接:http://dx.doi.org/10.1016/j.parint.2018.07.012

摘要

Little is known of the occurrence and age patterns of species/genotypes and subtypes of Cryptosporidium spp. and Giardia duodenalis in calves in Egypt. In this study, 248 fecal specimens were collected from dairy calves aged 1 day to 6 months on eight farms in three provinces during March 2015 to April 2016. Cryptosporidium spp. were detected and genotyped by using PCR-RFLP analysis of the small subunit rRNA (SSU rRNA) gene, while G. duodenalis was detected and genotyped by using PCR and sequence analyses of the triose phosphate isomerase (tpi), glutamate dehydrogenase (gdh) and beta-giardin (bg) genes. The overall infection rates of Cryptosporidium spp. and G. duodenalis were 9.7 and 13.3%, respectively. The highest Cryptosporidium infection rate (26.7%) was in calves of age <= 1 month while the highest G. duodenalis infection rate (44.4%) was in calves of 2 months. Three Cryptosporidium spp. were identified, including C. parvum (n = 16), C. bovis (n = 5) and C. ryanae (n = 3), with the former being almost exclusively found in calves of <= 3 months of age and the latter two being only found in calves of over 3 months. Subtyping of C. parvum by PCR-sequence analysis of the 60 kDa glycoprotein gene identified subtypes IIaA15G1R1 (n = 15) and IIaA15G2R1 (n = 1). The G. duodenalis identified included both assemblages E (n = 32) and A (n = 1), with the latter belonging to the anthroponotic subtype A2. These data provide new insights into the genetic diversity and age patterns of Cryptosporidium spp. and G. duodenalis in calves in Egypt.

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关键词
Cryptosporidium; Giardia duodenalis; Epidemiology; Genotypes; Subtypes; Cattle; Egypt

附加关键词:
MOLECULAR EPIDEMIOLOGY; UNITED-STATES; ASSEMBLAGE E; CATTLE; PREVALENCE; SPP.; GENOTYPES; ANIMALS; HUMANS; CYSTS

作者信息

通讯作者:
     Xiao, LH , South China Agr Univ, Coll Vet Med, Key Lab Zoonosis, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China.
作者机构:
     [1] [ Naguib, Doaa; El-Gohary, Adel H.; Mohamed, Amro A. ] Mansoura Univ, Dept Hyg & Zoonoses, Mansoura 35516, Egypt.
     [2] [ Naguib, Doaa; Roellig, Dawn M. ] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30329 USA.
     [3] [ Arafat, Nagah ] Mansoura Univ, Dept Poultry Dis, Mansoura 35516, Egypt.
     [4] [ Xiao, Lihua ] South China Agr Univ, Coll Vet Med, Key Lab Zoonosis, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China.
作者邮箱:
     lxiao1961@gmail.com